Early warning of faecal contamination of water: a dual mode, automated system for high- (< 1 hour) and low-levels (6-11 hours)

Water Sci Technol. 2001;43(12):217-20.

Abstract

There is a recognised need for methods that permit rapid estimation of the sanitary quality of water e.g. during raw water monitoring and emergencies involving water treatment failure or main breaks in a distribution network. In this study, two models for predicting the level of faecal contamination of water were studied. The first format, based on measurement of beta-galactosidase activity by the automated Colifast analyser, detected faecal contamination of high levels, corresponding to > 15 thermotolerant coliforms (FC)/5 mL, in 1-3 h, in a format that allowed for semi-quantification of results. By setting up a cut-off level, the system could be used as an operational tool identifying random increases in faecal contamination during routine raw water monitoring. A second Presence-Absence format was dependent upon the growth of low levels of FC with subsequent detection in the Colifast analyser. 95% of water samples containing 1-15 FC/sample volume showed positive detection after 11 h.

Publication types

  • Evaluation Study

MeSH terms

  • Automation
  • Biological Assay / methods
  • Enterobacteriaceae / isolation & purification*
  • Environmental Monitoring / methods
  • Feces*
  • Galactosidases / analysis
  • Galactosidases / metabolism*
  • Models, Theoretical*
  • Population Dynamics
  • Temperature
  • Water Purification
  • Water Supply*

Substances

  • Galactosidases