Suppression subtractive hybridization (SSH) was used to analyze differential expression of genes in the pigeon forebrain after the left retina of the pigeon was removed at hatching. Four genes out of the 14 identified clones produced positive hybridization signals as shown by reverse Northern blot. Sequencing results showed that the length of PFB/SSH-8 is 226 bp, of which 138 bp produced a homology of 85% with human CaMI; the length of PFB/SSH-15 is 252 bp, showing low homology with nexin I alpha; no matched homological information was found in the remaining 2 clones.