Abstract
From the aerial parts of Putoria calabrica, two new flavonol triglycosides were isolated and their structures were elucidated as quercetin-3-O-[alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside]-7-O-beta-D-glucopyranoside (1, calabricoside A) and quercetin-3-O-[4' "-O-caffeoyl-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-arabinopyranoside]-7-O-beta-D-glucopyranoside (2, calabricoside B). Additionally, seven iridoid and three lignan glycosides were isolated and characterized. Radical scavenging activities of all compounds were determined by quantifying their effects on luminol-enhanced chemiluminescence in formyl-methionyl-leucyl-phenylalanine (FMLP) stimulated human polymorphonuclear neutrophils (PMNs). Calabricoside A and B showed strong radical scavenging activity with IC(50) values of 0.25 and 0.3 microM, respectively.
MeSH terms
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Chromatography
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Esters / chemistry
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Flavonoids / chemistry
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Flavonoids / isolation & purification*
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Flavonoids / pharmacology
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Free Radical Scavengers / chemistry
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Free Radical Scavengers / isolation & purification*
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Free Radical Scavengers / pharmacology
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Glucosides / chemistry
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Glucosides / isolation & purification*
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Glucosides / pharmacology
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Humans
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Lignans / chemistry
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Lignans / isolation & purification*
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Lignans / pharmacology
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Luminescent Measurements
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Molecular Structure
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Plants, Medicinal / chemistry*
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Quercetin*
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Rubiaceae / chemistry*
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Spectrophotometry, Infrared
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Structure-Activity Relationship
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Turkey
Substances
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Esters
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Flavonoids
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Free Radical Scavengers
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Glucosides
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Lignans
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calabricoside A
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calabricoside B
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N-Formylmethionine Leucyl-Phenylalanine
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Quercetin