Abstract
The nucleocapsid (N) protein of subgroup C (United States-specific) avian pneumovirus (APV/US) was expressed in Escherichia coli, and antibodies to the recombinant N protein were shown to specifically recognize the approximately 47-kDa N protein of APV/US by Western immunoblot analysis. The recombinant APV/US N protein was used in a sandwich-capture enzyme-linked immunosorbent assay (ELISA), and the resulting assay was found to be more sensitive and specific than the routine indirect ELISA for the detection of APV/US antibodies in turkey sera.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood*
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Enzyme-Linked Immunosorbent Assay / methods
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Nucleocapsid Proteins / genetics
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Nucleocapsid Proteins / immunology*
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Nucleocapsid Proteins / metabolism
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Pneumovirus / immunology*
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Pneumovirus Infections / immunology
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Pneumovirus Infections / veterinary*
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Pneumovirus Infections / virology
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Poultry Diseases / immunology
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Poultry Diseases / virology*
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Rabbits
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / metabolism
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Turkeys*
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United States
Substances
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Antibodies, Viral
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Nucleocapsid Proteins
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Recombinant Proteins