Objective: To observe the effect of chondrocyte culture with natural ECM made from allogenous acellular cartilage.
Methods: The allogenous acellular cartilaginous matrix was used as a scaffold in chondrocyte culture in vitro, comparing with the acellular perichondrium. The growth and regeneration of the chondrocytes in vitro were observed.
Results: The chondrocytes seeded on the acellular cartilaginous matrix grew well while the chondrocytes on the acellular perichondrium matrix grew poorly.
Conclusion: The surface condition of the scaffold has a notable influence on the growth of the cells. The allogenous acellular cartilaginous matrix may provide a suitable condition for chondrocytes, and it has the possibility to be developed as a natural scaffold for tissue engineering.