Structural analysis of the exopolysaccharide from Burkholderia caribensis strain MWAP71

Carbohydr Res. 2001 Aug 23;334(2):127-33. doi: 10.1016/s0008-6215(01)00176-8.

Abstract

Burkholderia caribensis strain MWAP71 was isolated from rhizosphere soil microaggregates in Martinique. The extracellular polysaccharide produced by this strain was found to be composed of D-glucose (D-Glc), 6-deoxy-L-talose (L-6dTal), 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo), and an O-acetyl group in a molar ratio of 2:1:1:1. The primary structure of the polysaccharide was shown by sugar analysis, electrospray mass spectrometry, partial acid hydrolysis and 1-D and 2-D NMR spectroscopy to consist of a tetrasaccharide repeating unit having the following structure: [structure in text].

MeSH terms

  • Burkholderia / chemistry*
  • Deoxy Sugars / chemistry*
  • Glucose / chemistry*
  • Hexoses / chemistry*
  • Magnetic Resonance Spectroscopy
  • Polysaccharides, Bacterial / analysis
  • Polysaccharides, Bacterial / chemistry*
  • Soil Microbiology
  • Species Specificity
  • Spectrometry, Mass, Electrospray Ionization
  • Sugar Acids / chemistry*

Substances

  • Deoxy Sugars
  • Hexoses
  • Polysaccharides, Bacterial
  • Sugar Acids
  • 6-deoxytalose
  • Glucose
  • 3-deoxy-manno-oct-2-ulopyranosonic acid