Objective: To isolate arbovirus from mosquitoes caught from Yantai.
Methods: The isolated viruses were tested for their physico- chemical properties, examined by electron microscopy and specific immuno-reactivity.
Results: Fifteen strains of virus were isolated from mosquitoes in 1994 from Yantai. Three of them were further assayed. The results showed that the viruses could multiply on C6/36 cell and produce typical cytopathogenic effect. The viruses couldn't cause regular sickness and death of suckling mice by intracerebral inoculation. The viruses were sensitive to ether, but resistant to 5, -Idu. One sample was examined with electron microscope, and spherical virus particles were observed. The diameter of the virus particles is about 55 +/- 2. 3nm. It did not react with the JBE virus and Bunyaviridae group specific immuno-ascitic fluids but cross-reacted with the group A Togaviridae specific immuno-ascitic fluid.
Conclusions: The results showed that the viruses isolated from Yantai belong to alphavirus of togaviridae.