Abstract
Transcription factor Ets-1 expressed in endothelial cells promotes angiogenesis. Here, we transiently overexpressed Ets-1 in human umbilical vein endothelial cells (HUVECs) and comprehensively searched for potential downstream targets of Ets-1 by cDNA microarray analysis. The expression of several angiogenesis-related genes including neuropilin-1 was augmented by the overexpression of Ets-1. Quantitative real-time RT-PCR and Western blotting confirmed the increase in the levels of neuropilin-1 mRNA and protein. In contrast, dominant negative ets-1 decreased the levels of neuropilin-1 mRNA and protein. These results indicate that neuropilin-1 is a downstream target of Ets-1 in HUVECs.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Cells, Cultured
-
DNA Primers
-
DNA, Complementary
-
Endothelial Growth Factors / genetics
-
Endothelial Growth Factors / metabolism*
-
Endothelium, Vascular / cytology
-
Endothelium, Vascular / metabolism*
-
Humans
-
Lymphokines / genetics
-
Lymphokines / metabolism*
-
Oligonucleotide Array Sequence Analysis
-
Protein Binding
-
Proto-Oncogene Protein c-ets-1
-
Proto-Oncogene Proteins / metabolism*
-
Proto-Oncogene Proteins c-ets
-
Transcription Factors / metabolism*
-
Umbilical Veins / cytology
-
Umbilical Veins / metabolism*
-
Vascular Endothelial Growth Factor A
-
Vascular Endothelial Growth Factors
Substances
-
DNA Primers
-
DNA, Complementary
-
ETS1 protein, human
-
Endothelial Growth Factors
-
Lymphokines
-
Proto-Oncogene Protein c-ets-1
-
Proto-Oncogene Proteins
-
Proto-Oncogene Proteins c-ets
-
Transcription Factors
-
Vascular Endothelial Growth Factor A
-
Vascular Endothelial Growth Factors