Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses

K E Van Vliet; P Muir; J M Echevarria; P E Klapper; G M Cleator; A M Van Loon

doi:10.1128/JCM.39.9.3390-3392.2001

Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses

J Clin Microbiol. 2001 Sep;39(9):3390-2. doi: 10.1128/JCM.39.9.3390-3392.2001.

Authors

K E Van Vliet¹, P Muir, J M Echevarria, P E Klapper, G M Cleator, A M Van Loon

Affiliation

¹ Department of Virology, University Medical Center Utrecht, Utrecht, The Netherlands.

Abstract

A multicenter study of molecular detection of enteroviruses was conducted using a proficiency panel. Of 70 data sets, 46 (66%) reported correct results for samples containing at least 1 50% infective dose per ml and for negative samples. Variation in performance between laboratories demonstrates the need for ongoing quality control.

Publication types

Multicenter Study
Research Support, Non-U.S. Gov't

MeSH terms

Enterovirus / genetics
Enterovirus / isolation & purification*
Enterovirus Infections / diagnosis*
Enterovirus Infections / virology
Humans
Laboratories / standards*
Nucleic Acid Amplification Techniques / methods*
Nucleic Acid Amplification Techniques / standards
Quality Control
RNA, Viral / analysis

Substances

RNA, Viral