Psychophysiological assessment of pilot mental workload using heart rate should be augmented with an autonomic space model of cardiovascular function. This model proposes that autonomic nervous system influences on the heart may change with psychological processing in ways that are not evident in heart rate. A method of mental-workload assessment was proposed that used multiple psychophysiological measures of cardiovascular responsivity to derive the underlying sympathetic and parasympathetic information needed to represent the autonomic space for heart rate. Principal-components analysis was used to extract Sympathetic and Parasympathetic components from heart period, residual heart period. respiratory sinus arrhythmia, and Traube-Hering-Mayer wave in three experiments that manipulated perceptual/central processing and physical task demands. This initial evaluation of the method concluded that the autonomic components were valid and that the components had greater diagnosticity, and for some manipulations greater sensitivity, than heart rate. These results support the contention that the Sympathetic and Parasympathetic components provided increased precision for mental-workload assessment.