Recent progress in the noninvasive evaluation methods of cardiovascular autonomic regulation, including autonomic function tests, baroreflex function assessment, and heart rate, blood pressure variability analysis were discussed, and the results of applying these methods in physiological studies on head-down bed rest, head-up tilt, lower body negative pressure and aerobic training were reported. It suggests that noninvasive evaluation methods of cardiovascular autonomic regulation are effective means in weightlessness physiology study.