Abstract
Although tumor necrosis factor receptor-associated factor 6 (TRAF6) is required in receptor activator of NF-kappaB-receptor activator of NF-kappaB ligand (RANK-RANKL) signaling for osteoclastogenesis, it has remained unclear whether TRAF6 is crucial in tumor necrosis factor alpha (TNF-alpha)-induced osteoclastogenesis. We examined TRAF6 function in the TNF-alpha-induced osteoclastogenesis by using osteoclast progenitor cells from TRAF6-deficient mice. The results indicated that TNF-alpha did not effectively induce osteoclast differentiation from osteoclast progenitor cells derived from these mice into mature multinucleated osteoclasts, although c-jun N-terminal kinase (JNK) and TNF-alpha activation was observed in osteoclast progenitor cells. Thus, we have provided the first evidence showing that TRAF6 is involved in TNF-alpha-induced osteoclastogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Differentiation / drug effects
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Cells, Cultured
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JNK Mitogen-Activated Protein Kinases
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Mice
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Mice, Knockout
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Mitogen-Activated Protein Kinases / metabolism
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NF-kappa B / metabolism
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Osteoblasts / cytology*
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Osteoblasts / drug effects
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Osteoblasts / metabolism
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Osteogenesis / physiology*
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Proteins / genetics
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Proteins / metabolism*
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Proteins / physiology
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Receptors, Tumor Necrosis Factor / metabolism*
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Signal Transduction*
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Stem Cells / cytology*
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Stem Cells / drug effects
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Stem Cells / metabolism
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TNF Receptor-Associated Factor 6
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Tumor Necrosis Factor-alpha / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology
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Up-Regulation
Substances
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NF-kappa B
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Proteins
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Receptors, Tumor Necrosis Factor
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TNF Receptor-Associated Factor 6
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Tumor Necrosis Factor-alpha
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases