STRICA, a novel Drosophila melanogaster caspase with an unusual serine/threonine-rich prodomain, interacts with DIAP1 and DIAP2

Cell Death Differ. 2001 Apr;8(4):387-94. doi: 10.1038/sj.cdd.4400864.

Abstract

The recently published genome sequence of Drosophila melanogaster predicts seven caspases in the fly. Five of these caspases have been previously characterised. Here, we describe the Drosophila caspase, STRICA. STRICA is a caspase with a long amino-terminal prodomain that lacks any caspase recruitment domain or death effector domain. Instead, the prodomain of STRICA consists of unique serine/threonine stretches. Low levels of strica expression were detected in embryos, larvae, pupae and adult animals. STRICA is a cytoplasmic protein that, upon overexpression, caused apoptosis in cultured Drosophila SL2 cells that was partially suppressed by DIAP1. Interestingly, unlike other fly caspases, STRICA showed physical association with DIAP2, in cotransfection experiments. These results suggest that STRICA may have a unique cellular function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis*
  • Caspases / genetics*
  • Caspases / metabolism*
  • Caspases / physiology*
  • Cell Line
  • Cloning, Molecular
  • Dimerization
  • Drosophila Proteins*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / enzymology*
  • Inhibitor of Apoptosis Proteins
  • Insect Proteins / metabolism*
  • Molecular Sequence Data
  • Phylogeny
  • Protein Structure, Tertiary
  • RNA, Messenger / biosynthesis
  • Serine / chemistry
  • Threonine / chemistry
  • Transfection
  • Two-Hybrid System Techniques

Substances

  • DIAP1 protein, Drosophila
  • DIAP2 protein, Drosophila
  • Drosophila Proteins
  • Inhibitor of Apoptosis Proteins
  • Insect Proteins
  • RNA, Messenger
  • Threonine
  • Serine
  • Caspases
  • STRICA protein, Drosophila

Associated data

  • GENBANK/AF242735