Abstract
The fur gene of Pasteurella multocida has been cloned by complementation of an Escherichia coli fur mutant. The P. multocida fur gene, which encodes a predicted protein of 147 amino acids, displaying the highest identity (89%) with the same protein of Haemophilus influenzae, is negatively regulated by its own product. By construction of a P. multocida fur mutant, it has been demonstrated that the ompH gene, encoding a major structural protein of the outer membrane, presenting high antigenicity power, is negatively regulated by iron and glucose. Furthermore, wild-type and fur-defective cells of P. multocida show the same level of virulence.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Outer Membrane Proteins / genetics*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Down-Regulation
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Gene Expression Regulation, Bacterial* / drug effects
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Glucose / pharmacology
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Iron / pharmacology
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Mice
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Molecular Sequence Data
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Mutation
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Pasteurella multocida / genetics*
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Pasteurella multocida / pathogenicity
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Virulence
Substances
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Repressor Proteins
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ferric uptake regulating proteins, bacterial
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ompH protein, bacteria
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Iron
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Glucose