Synthesis and evaluation of alpha-LNA

N K Christensen; J K Dalskov; P Nielsen

doi:10.1081/NCN-100002438

Synthesis and evaluation of alpha-LNA

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):825-8. doi: 10.1081/NCN-100002438.

Authors

N K Christensen¹, J K Dalskov, P Nielsen

Affiliation

¹ Department of Chemistry, University of Southern Denmark, 5230 Odense M, Denmark.

PMID: 11563124
DOI: 10.1081/NCN-100002438

Abstract

Three different synthetic routes to the alpha-configured LNA thymine monomer starting from D-allose or D-arabinose were investigated. The introduction of one or four alpha-LNA monomers into alpha-DNA had a destabilizing effect on the duplexes. However, a fully modified alpha-LNA sequence displayed strong recognition of complementary RNA, but no transition with DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabinose / chemistry
DNA / chemistry
Glucose / chemistry
Nucleic Acid Conformation
Oligonucleotides / chemical synthesis*
Oligonucleotides / chemistry
RNA / chemistry
Thymine / analogs & derivatives
Thymine / chemistry

Substances

Oligonucleotides
allose
RNA
DNA
Arabinose
Glucose
Thymine