Cutting edge: STAT activation by IL-19, IL-20 and mda-7 through IL-20 receptor complexes of two types

J Immunol. 2001 Oct 1;167(7):3545-9. doi: 10.4049/jimmunol.167.7.3545.

Abstract

IL-10-related cytokines include IL-20 and IL-22, which induce, respectively, keratinocyte proliferation and acute phase production by hepatocytes, as well as IL-19, melanoma differentiation-associated gene 7, and AK155, three cytokines for which no activity nor receptor complex has been described thus far. Here, we show that mda-7 and IL-19 bind to the previously described IL-20R complex, composed by cytokine receptor family 2-8/IL-20Ralpha and DIRS1/IL-20Rbeta (type I IL-20R). In addition, mda-7 and IL-20, but not IL-19, bind to another receptor complex, composed by IL-22R and DIRS1/IL20Rbeta (type II IL-20R). In both cases, binding of the ligands results in STAT3 phosphorylation and activation of a minimal promoter including STAT-binding sites. Taken together, these results demonstrate that: 1) IL-20 induces STAT activation through IL-20R complexes of two types; 2) mda-7 and IL-20 redundantly signal through both complexes; and 3) IL-19 signals only through the type I IL-20R complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carrier Proteins / metabolism
  • Cell Line
  • DNA-Binding Proteins / metabolism*
  • Genes, Reporter
  • Genes, Tumor Suppressor
  • Growth Substances / pharmacology*
  • Humans
  • Interleukin-10 / pharmacology*
  • Interleukin-22
  • Interleukins / pharmacology*
  • Macromolecular Substances
  • Models, Biological
  • Receptors, Cell Surface*
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin / metabolism*
  • STAT3 Transcription Factor
  • Signal Transduction
  • Trans-Activators / metabolism*
  • Transcriptional Activation
  • Transfection

Substances

  • Carrier Proteins
  • DNA-Binding Proteins
  • Growth Substances
  • IL19 protein, human
  • IL22RA2 protein, human
  • Interleukins
  • Macromolecular Substances
  • Receptors, Cell Surface
  • Receptors, Interleukin
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • Trans-Activators
  • interleukin-22 receptor
  • interleukin-24
  • Interleukin-10
  • interleukin 20