Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit

B Böddinghaus; T A Wichelhaus; V Brade; T Bittner

doi:10.1128/JCM.39.10.3750-3752.2001

Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit

J Clin Microbiol. 2001 Oct;39(10):3750-2. doi: 10.1128/JCM.39.10.3750-3752.2001.

Authors

B Böddinghaus¹, T A Wichelhaus, V Brade, T Bittner

Affiliation

¹ Institute of Medical Microbiology, University Hospital, Frankfurt am Main, Germany. [email protected]

Abstract

The effectiveness of PCR inhibitor removal by silica membranes in combination with the Amplicor Mycobacterium tuberculosis kit was analyzed for 655 respiratory and nonrespiratory specimens. The overall inhibition rate was reduced from 12.5%, when applying the Amplicor kit alone, to 1.1% with the addition of silica membrane DNA purification.

Publication types

Evaluation Study

MeSH terms

Culture Media
DNA, Bacterial / isolation & purification*
Filtration / methods
Humans
Membranes, Artificial*
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification*
Polymerase Chain Reaction / methods*
Reagent Kits, Diagnostic
Sensitivity and Specificity
Silicon Dioxide*
Tuberculosis / microbiology

Substances

Culture Media
DNA, Bacterial
Membranes, Artificial
Reagent Kits, Diagnostic
Silicon Dioxide