Abstract
Rotavirus strains collected in the United Kingdom during the 1995-1996 season and genotyped as G2 by reverse transcription-PCR failed to serotype in enzyme-linked immunosorbent assays using three different G2-specific monoclonal antibodies. The deduced amino acid sequences of the antigenic regions A (amino acids 87 to 101), B (amino acids 142 to 152), and C (amino acids 208 to 221) of VP7 revealed that a substitution at position 96 (Asp-->Asn) correlated with the change in ability to serotype these G2 strains.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution*
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Antibodies, Monoclonal / immunology
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Antigens, Viral*
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Capsid / chemistry*
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Capsid / genetics*
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Capsid / immunology
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Capsid Proteins*
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Enzyme-Linked Immunosorbent Assay
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Humans
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Molecular Sequence Data
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Reverse Transcriptase Polymerase Chain Reaction
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Rotavirus / classification*
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Rotavirus / genetics
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Rotavirus Infections / virology
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Serotyping
Substances
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Antibodies, Monoclonal
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Antigens, Viral
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Capsid Proteins
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VP7 protein, Rotavirus