In-vitro and in-vivo immunomodulatory effects of syringin

J Pharm Pharmacol. 2001 Sep;53(9):1287-94. doi: 10.1211/0022357011776577.

Abstract

Syringin was found to possess immunomodulatory activity by which it inhibited the in-vitro immunohaemolysis of antibody-coated sheep erythrocytes by guinea-pig serum through suppression of C3-convertase of the classical complement. In this study, we examined its in-vitro and in-vivo activity on tumour necrosis factor (TNF)-alpha and nitric oxide (NO) production, CD4+ T cell and CD8+ cytotoxic T cell (CTLL-2) proliferation, and croton oil-, arachidonic acid- and fluorescein-isothiocynate (FITC)-induced mouse ear oedema model. Syringin significantly inhibited both TNF-alpha production from lipopolysaccharide (LPS)-stimulated RAW264.7 cells and CD8+ T cell (CTLL-2) proliferation in a dose-dependent manner, whereas neither NO production nor CD4+ T cell proliferation were blocked even by high concentrations of syringin. In the invivo experiments, syringin also significantly suppressed FITC-induced ear oedema in mice but not the ear oedema induced by croton or arachidonic acid. These results suggest that syringin may be implicated as an immunomodulator having an anti-allergic effect rather than an anti-inflammatory effect. The anti-allergic effect of syringin seems to be due, in part, to inhibition of TNF-alpha production and cytotoxic T cell proliferation.

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Cell Culture Techniques
  • Cell Division / drug effects
  • Glucosides / immunology*
  • Glucosides / pharmacology*
  • Guinea Pigs
  • Immune System / drug effects
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred ICR
  • Phenylpropionates / immunology*
  • Phenylpropionates / pharmacology*
  • Sheep
  • T-Lymphocytes, Cytotoxic / drug effects*
  • Tumor Necrosis Factor-alpha / biosynthesis
  • Tumor Necrosis Factor-alpha / drug effects*

Substances

  • Adjuvants, Immunologic
  • Glucosides
  • Phenylpropionates
  • Tumor Necrosis Factor-alpha
  • syringin