Chiral analysis of methylphenidate and dextromoramide by capillary electrophoresis

J Chromatogr B Biomed Sci Appl. 2001 Sep 15;761(1):61-8. doi: 10.1016/s0378-4347(01)00300-0.

Abstract

Capillary electrophoretic methods have been developed to separate the enantiomers of methylphenidate (MPH) and dextromoramide. For MPH separation was achieved with heptakis (2,6-di-O-methyl)-beta-cyclodextrin (DMCD) as chiral selector in a 100 mM phosphoric acid buffer adjusted to pH 3.0 with triethanolamine. Commercial samples of D,L-erytho-MPH HCl and D,L-threo-MPH HCl were analysed using the method. There was no evidence of the presence of D,L-threo-MPH HCl in D,L-erytho-MPH HCl and vice versa. The ratio of the enantiomers was determined for each diastereoisomer. Hydroxypropyl-beta-cyclodextrin was the chiral selector of choice for the chiral separation of the enantiomers of moramide. The separation which gave a resolution of about 3.5 was achieved in 4 min using only a 6 cm of length of capillary. In a sample of dextro-R-moramide tartrate only a small quantity (4.9% w/w) of levo-S-moramide was detected with this method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Buffers
  • Dextromoramide / analysis*
  • Electrophoresis, Capillary / methods*
  • Methylphenidate / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Stereoisomerism

Substances

  • Buffers
  • Methylphenidate
  • Dextromoramide