Abstract
We have isolated a cDNA encoding a splice variant of ASIC (acid-sensing ion channel)-beta from the rat trigeminal ganglion. This clone, designated ASIC-beta2, showed a 342 base deletion just after the first transmembrane domain in ASIC-beta. RT-PCR experiments revealed that ASIC-beta2 was expressed exclusively in the trigeminal ganglion and dorsal root ganglion. In situ hybridization showed that ASIC-beta2 mRNA was concentrated in both small diameter and large diameter neurons and co-localized with ASIC-beta mRNA within single sensory neurons in the trigeminal ganglion. When expressed in Xenopus oocytes, ASIC-beta2 was inactive by itself. However, it associated with ASIC-beta to form heteromers, which display lower affinity for protons than ASIC-beta alone.
MeSH terms
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Acid Sensing Ion Channels
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Alternative Splicing / physiology*
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Amino Acid Sequence / physiology
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Animals
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Base Sequence / physiology
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Cell Size / genetics
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Cloning, Molecular
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DNA, Complementary / isolation & purification*
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Ganglia, Sensory / cytology
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Ganglia, Sensory / metabolism*
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Gene Expression / physiology*
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Membrane Proteins*
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Molecular Sequence Data
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Nerve Tissue Proteins*
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Oocytes / metabolism
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Pain / metabolism*
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Pain / physiopathology
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Protein Isoforms / genetics
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Sodium Channels / genetics*
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Trigeminal Ganglion / cytology
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Trigeminal Ganglion / metabolism*
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Xenopus laevis
Substances
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ASIC3 protein, human
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Acid Sensing Ion Channels
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DNA, Complementary
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Membrane Proteins
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Nerve Tissue Proteins
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Protein Isoforms
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RNA, Messenger
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Sodium Channels
Associated data
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GENBANK/AB049451
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GENBANK/AJ006519