Differential effect of IL-4 and IL-13 on CD44 expression in the Burkitt's lymphoma B cell line BL30/B95-8 and in Epstein-Barr virus (EBV) transformed human B cells: loss of IL-13 receptors on Burkitt's lymphoma B cells

Cell Immunol. 2001 Aug 1;211(2):131-42. doi: 10.1006/cimm.2001.1829.

Abstract

IL-4 and IL-13, cytokines with similar biological effects may influence growth and progression of B-cell tumors through regulation of key cell surface molecules important in intercellular communications. In this study, we demonstrate that IL-4 and IL-13 exhibited differential effects on CD23 and CD44 expression and binding to hyaluronan in BL30/B95-8, a Burkitt's lymphoma (BL), and MK3.31, an Epstein-Barr virus transformed normal human B cell line (B-LCL). Studies conducted to understand the molecular mechanisms underlying this differential effect show that IL-4 induced phosphorylation of JAK1, JAK3, and STAT6 in BL30/B95-8 cells and of JAK3 and STAT6 in MK 3.31 cells. In contrast, IL-13 failed to induce the phosphorylation of JAK kinases or STAT6 proteins in these cell lines. The inability of BL30/B95-8 cells to respond to IL-13 was attributed to the loss of expression of IL-13R subunits alpha1 and alpha2, a finding confirmed for a number of other BL cell lines examined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • Burkitt Lymphoma
  • Cell Line, Transformed
  • Enzyme Activation
  • Gene Expression
  • Herpesvirus 4, Human / genetics
  • Humans
  • Hyaluronan Receptors / biosynthesis*
  • Hyaluronan Receptors / metabolism
  • Hyaluronic Acid / metabolism
  • Interleukin-13 / immunology*
  • Interleukin-13 / pharmacology
  • Interleukin-13 Receptor alpha1 Subunit
  • Interleukin-4 / immunology*
  • Interleukin-4 / pharmacology
  • Janus Kinase 1
  • Janus Kinase 3
  • Phosphorylation
  • Protein Binding
  • Protein-Tyrosine Kinases / metabolism
  • Receptors, IgE / biosynthesis
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin / immunology*
  • Receptors, Interleukin-13
  • STAT6 Transcription Factor
  • Signal Transduction
  • Trans-Activators / metabolism
  • Tumor Cells, Cultured

Substances

  • Hyaluronan Receptors
  • IL13RA1 protein, human
  • Interleukin-13
  • Interleukin-13 Receptor alpha1 Subunit
  • Receptors, IgE
  • Receptors, Interleukin
  • Receptors, Interleukin-13
  • STAT6 Transcription Factor
  • STAT6 protein, human
  • Trans-Activators
  • Interleukin-4
  • Hyaluronic Acid
  • Protein-Tyrosine Kinases
  • JAK1 protein, human
  • JAK3 protein, human
  • Janus Kinase 1
  • Janus Kinase 3