Abstract
Streptococcus pyogenes secretes a specific immunoglobulin G (IgG)-protease, SpeB, as well as the IgG glycan-hydrolyzing enzyme EndoS. Here we show that SpeB also degrades IgA, IgM, IgD, and IgE. We also show that EndoS only hydrolyzes the glycan moiety on native but not denatured IgG. Thus, SpeB has a broad immunoglobulin-degrading activity, while EndoS is highly specific for IgG.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Bacterial / metabolism*
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Bacterial Proteins / metabolism*
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Cysteine Endopeptidases / metabolism*
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Glycoside Hydrolases / metabolism*
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Humans
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Immunoglobulin A / metabolism
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Immunoglobulin D / metabolism
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Immunoglobulin E / metabolism
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Immunoglobulin G / metabolism
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Immunoglobulin M / metabolism
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Immunoglobulins / metabolism*
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Streptococcus pyogenes / enzymology*
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Substrate Specificity
Substances
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Antibodies, Bacterial
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Bacterial Proteins
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Immunoglobulin A
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Immunoglobulin D
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Immunoglobulin G
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Immunoglobulin M
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Immunoglobulins
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Immunoglobulin E
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Glycoside Hydrolases
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NDOS protein, Streptococcus pyogenes
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Cysteine Endopeptidases
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streptopain