Detection of Fcgamma binding protein antigen in human sera and its relation with autoimmune diseases

Immunol Lett. 2001 Dec 3;79(3):229-35. doi: 10.1016/s0165-2478(01)00288-7.

Abstract

Intestinal goblet cells of patients with ulcerative colitis and Crohn's disease highly express a binding protein of the Fc portion of IgG (FcgammaBP), which is entirely different from the Fcgamma receptors I,II, and III on neutrophils and macrophages. In this study, we proved the qualitative existence of FcgammaBP antigen in the sera of patients with rheumatoid arthritis and systemic lupus erythematosus, and, further, established a highly sensitive and quantitatively reproducible assay for FcgammaBP antigen in order to prevent the cross-reactivity of FcgammaBP with von Willebrand factor which has about 30% homology. This assay revealed a higher level of FcgammaBP antigen in the blood stream of patients with autoimmune diseases, especially progressive systemic sclerosis. This would suggest that abnormal production of autoantibodies reflects increased generation of FcgammaBP in goblet cells and its secretion into the circulation by an unknown mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Arthritis, Rheumatoid / blood
  • Arthritis, Rheumatoid / immunology
  • Autoantigens / blood
  • Autoantigens / immunology*
  • Autoimmune Diseases / blood
  • Autoimmune Diseases / immunology*
  • CHO Cells
  • Carrier Proteins / blood
  • Carrier Proteins / immunology*
  • Cell Adhesion Molecules
  • Cricetinae
  • Cross Reactions / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Haplorhini
  • Humans
  • Immune Sera / immunology
  • Lupus Erythematosus, Systemic / blood
  • Lupus Erythematosus, Systemic / immunology
  • Membrane Proteins
  • Precipitin Tests
  • Rabbits
  • Reproducibility of Results
  • Sensitivity and Specificity
  • von Willebrand Factor / immunology

Substances

  • Antibodies, Monoclonal
  • Autoantigens
  • Carrier Proteins
  • Cell Adhesion Molecules
  • FCGBP protein, human
  • Immune Sera
  • Membrane Proteins
  • von Willebrand Factor