Abstract
Dendritic cells are crucial to the activation as well as suppression of the immune response. Previous reports have illustrated that APC interacting with antigen-specific T suppressor cells become tolerogenic, inducing T helper anergy. To characterize the molecular changes occurring in tolerogenic APC, the mRNA profile of KG-1 dendritic cells exposed to allospecific T helper and T suppressor cells were analyzed. This study now provides evidence that immature dendritic cells stimulated by T suppressor cells differentiate into mature dendritic cells with a distinct phenotype. The identification of Ts induced pathways of dendritic cell differentiation is critical to the development of new therapeutic strategies.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, Differentiation
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Apoptosis / genetics
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Apoptosis / immunology
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Cell Communication / genetics
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Cell Communication / immunology
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Cell Cycle Proteins
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Cell Division / genetics
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Cell Division / immunology
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Cell Line
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Chemokines / biosynthesis
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Chemokines / genetics
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Chemokines / metabolism
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Coculture Techniques
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Cyclooxygenase 2
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Cytokines / biosynthesis
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Cytokines / genetics
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Dendritic Cells / cytology
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Dendritic Cells / enzymology
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism
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Down-Regulation / genetics
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Down-Regulation / immunology
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Gene Expression Profiling* / methods
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Humans
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Immune Tolerance* / genetics
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Isoenzymes / biosynthesis
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Isoenzymes / genetics
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Lymphocyte Activation / genetics
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Membrane Proteins
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Oligonucleotide Array Sequence Analysis* / methods
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Prostaglandin-Endoperoxide Synthases / biosynthesis
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Prostaglandin-Endoperoxide Synthases / genetics
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Protein Biosynthesis
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Protein Phosphatase 1
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Proteins / genetics
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RNA, Messenger / genetics*
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Signal Transduction / genetics
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Signal Transduction / immunology
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T-Lymphocytes, Helper-Inducer / immunology
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T-Lymphocytes, Regulatory / immunology
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Tumor Cells, Cultured
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Up-Regulation / genetics
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Up-Regulation / immunology
Substances
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Antigens, Differentiation
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Cell Cycle Proteins
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Chemokines
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Cytokines
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Isoenzymes
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Membrane Proteins
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Proteins
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RNA, Messenger
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Transcription Factors
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Cyclooxygenase 2
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PTGS2 protein, human
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Prostaglandin-Endoperoxide Synthases
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PPP1R15A protein, human
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Protein Phosphatase 1