Abstract
BMS-284756, a novel des-fluoro(6)-quinolone, was used to select for in vitro mutants of Staphylococcus aureus ISP794. Step mutants were obtained, and the quinolone resistance-determining regions of four target genes, gyrA, gyrB, grlA, and grlB, were sequenced. The data suggest that DNA gyrase is the primary target for BMS-284756 in S. aureus.
MeSH terms
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Anti-Infective Agents / pharmacology*
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Ciprofloxacin / pharmacology
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DNA Gyrase / drug effects
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DNA Gyrase / genetics
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Drug Resistance, Microbial
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Fluoroquinolones*
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Indoles*
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Mutation / genetics
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Quinolones*
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Reverse Transcriptase Polymerase Chain Reaction
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / metabolism*
Substances
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Anti-Infective Agents
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DNA, Bacterial
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Fluoroquinolones
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Indoles
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Quinolones
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Ciprofloxacin
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DNA Gyrase
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garenoxacin