[The effects of 6A8 cDNA transfection on biological behavior of tumor cells]

Zhonghua Yi Xue Za Zhi. 1999 Apr;79(4):292-4.
[Article in Chinese]

Abstract

Objective: To investigate the effects of antisense 6A8 cDNA on the malignant behavior of tumor.

Methods: Highly metastatic clonal variant CNE-2L2 from nasopharygeal carcinoma cell line(CNE-2Z) was transfected with antisense 6A8 cDNA or mock plasmid. Their adhesion, locomotion, proteolytic ability, in vitro invasion, in vivo growth and metastasis were comparatively studied with the wild CNE-2L2 cells.

Results: Antisense 6A8 cDNA transfected CNE-2L2 showed less adhesion to FN and LN, weakened locomotive ability and proteolytic ability, retarded in vivo growth and much less metastasis.

Conclusion: Transfection of antisense 6A8 cDNA obviously decreased the malignant behavior of tumor cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion
  • DNA, Antisense / genetics*
  • DNA, Complementary / genetics*
  • Humans
  • Nasopharyngeal Neoplasms / pathology*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • DNA, Antisense
  • DNA, Complementary