Abstract
An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4alpha2 (HNF4alpha2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4alpha2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, alpha1-antitrypsin, and HNF1alpha were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.
MeSH terms
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Apolipoprotein A-I / genetics
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Apolipoprotein C-II
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Apolipoprotein C-III
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Apolipoproteins C / genetics
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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Blotting, Northern
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Cell Line
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Cell Line, Transformed
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DNA-Binding Proteins*
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Down-Regulation
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Electrophoresis, Polyacrylamide Gel
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Electrophoretic Mobility Shift Assay
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Factor X / genetics
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Female
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Gene Expression Regulation
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-alpha
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Hepatocyte Nuclear Factor 1-beta
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Hepatocyte Nuclear Factor 4
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Hepatocytes / metabolism*
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Humans
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Liver / cytology
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Liver / metabolism*
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Male
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Nuclear Proteins*
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Oligonucleotides / metabolism
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Phosphoproteins / genetics
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Phosphoproteins / metabolism
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Phosphoproteins / physiology*
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Protein Binding
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcription Factors / physiology*
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Transfection
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Tumor Cells, Cultured
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alpha 1-Antitrypsin / genetics
Substances
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Apolipoprotein A-I
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Apolipoprotein C-II
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Apolipoprotein C-III
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Apolipoproteins C
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Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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HNF1A protein, human
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HNF1B protein, human
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Hepatocyte Nuclear Factor 1-alpha
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Hepatocyte Nuclear Factor 4
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MLX protein, human
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Nuclear Proteins
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Oligonucleotides
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Phosphoproteins
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RNA, Messenger
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Transcription Factors
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alpha 1-Antitrypsin
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apolipoprotein C-II (St Michael)
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-beta
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Factor X