Adociasulfates 1-6, Inhibitors of Kinesin Motor Proteins from the Sponge Haliclona (aka Adocia) sp

Christine L. Blackburn; Cordula Hopmann; Roman Sakowicz; Michael S. Berdelis; Lawrence S. B. Goldstein; D. John Faulkner

doi:10.1021/jo9824448

Adociasulfates 1-6, Inhibitors of Kinesin Motor Proteins from the Sponge Haliclona (aka Adocia) sp

J Org Chem. 1999 Jul 23;64(15):5565-5570. doi: 10.1021/jo9824448.

Authors

Christine L. Blackburn¹, Cordula Hopmann, Roman Sakowicz, Michael S. Berdelis, Lawrence S. B. Goldstein, D. John Faulkner

Affiliation

¹ Scripps Institution of Oceanography, University of California at San Diego, La Jolla, California 92093-0212, and Department of Pharmacology, Division of Cellular and Molecular Medicine, Howard Hughes Medical Institute, University of California at San Diego, La Jolla, California 92093-0683.

PMID: 11674622
DOI: 10.1021/jo9824448

Abstract

Adociasulfates 1-6 (1-6) were isolated from an extract of the Palauan sponge Haliclona (aka Adocia) sp. that inhibited the transport of stabilized microtubules by the motor protein kinesin, which was immobilized on a microscope slide. The structures of adociasulfates 1-6, the relative stereochemistry of adociasulfates 1, 2, 5, and 6, and the relative stereochemistry of subunits of adociasulfates 3 and 4 were determined by interpretation of spectroscopic data. In a quantitative assay that measures ATP hydrolysis by kinesin, adociasulfates 2 and 6 were the most active.