Abstract
Map and Eap are secreted Staphylococcus aureus proteins that interact with various extracellular matrix molecules. PCR analysis using map primers yielded positive reactions in 97.9% of S. aureus isolates but not in Staphylococcus epidermidis isolates. Cloning and sequencing of the conferring genes revealed a high degree of overall homology combined with size variability of the gene product due to various repeat numbers and early translation termination in a poly(A) region. Thus, Map and Eap may provide a potential novel tool for S. aureus identification and typing.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, Bacterial / genetics
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Bacterial Proteins / genetics*
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Conserved Sequence
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DNA Primers
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Gene Expression Regulation, Bacterial
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Humans
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Molecular Sequence Data
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Polymorphism, Genetic
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RNA-Binding Proteins / genetics*
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Sequence Analysis, DNA
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Staphylococcal Infections / microbiology*
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Staphylococcus aureus / genetics*
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Staphylococcus epidermidis
Substances
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Antigens, Bacterial
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Bacterial Proteins
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DNA Primers
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Eap-N protein, Staphylococcus aureus
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RNA-Binding Proteins
Associated data
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GENBANK/AJ243790
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GENBANK/AJ245439
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GENBANK/AJ290973