Abstract
Transcription of the interferon regulatory factor-1 gene (IRF-1) is induced in a biphasic manner (G1 and G1/S phase) in Nb2 T cells in response to prolactin (PRL) stimulation. Signal transducer and activator of transcription 1 (Stat1) is required for PRL activation of the IRF-1 promoter. Mutation of a -200 bp Sp1 site in the IRF-1 promoter results in a loss of G1 but not G1/S IRF-1 transcriptional activity in response to PRL. These studies illustrate that the temporal transcription of the IRF-1 gene is mediated by not only Stat1 but also Sp1 in response to PRL stimulation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Binding Sites
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DNA-Binding Proteins / drug effects
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology
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G1 Phase
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Interferon Regulatory Factor-1
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Mutation
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Phosphoproteins / drug effects
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Phosphoproteins / genetics*
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Prolactin / metabolism
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Prolactin / pharmacology*
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Prolactin / physiology
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Promoter Regions, Genetic
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Rats
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STAT1 Transcription Factor
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Signal Transduction
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Sp1 Transcription Factor / genetics
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Sp1 Transcription Factor / metabolism
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Sp1 Transcription Factor / physiology*
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Trans-Activators / drug effects
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Trans-Activators / metabolism
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Trans-Activators / physiology
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Transcriptional Activation / drug effects*
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Transfection
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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Interferon Regulatory Factor-1
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Irf1 protein, rat
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Phosphoproteins
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STAT1 Transcription Factor
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Sp1 Transcription Factor
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Stat1 protein, rat
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Trans-Activators
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Prolactin