Abstract
A chimera of the two immunodominant African swine fever (ASF) virus proteins p54 and p30 was constructed by insertion of the gene CP204L into a Not I restriction site of E183L gene. The resulting chimeric protein p54/30, expressed by a recombinant baculovirus in insect cells and in Trichoplusia ni larvae, retained antigenic determinants present in both proteins and reacted in Western blot with a collection of sera from inapparent ASF virus carrier pigs. Remarkably, pigs immunized with the chimeric protein developed neutralizing antibodies and survived the challenge with a virulent African swine fever virus, presenting a reduction of about two logs in maximum viremia titers with respect to control pigs. In conclusion, this study revealed that the constructed chimeric protein may have utility as a serological diagnostic reagent and for further immunological studies that may provide new insights on mechanisms of protective immunity to ASFV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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African Swine Fever / diagnosis*
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African Swine Fever / prevention & control*
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African Swine Fever / virology
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African Swine Fever Virus / immunology*
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Animals
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Antibodies, Viral / blood
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Baculoviridae / genetics
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Baculoviridae / metabolism
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Cells, Cultured
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Immunoblotting
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Immunodominant Epitopes* / genetics
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Immunodominant Epitopes* / immunology*
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Immunodominant Epitopes* / metabolism
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Macrophages, Alveolar / virology
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Moths / virology
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Neutralization Tests
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Phosphoproteins / genetics
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Phosphoproteins / immunology*
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Phosphoproteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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Spodoptera
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Swine
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Vaccination
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Viral Proteins / genetics
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Viral Proteins / immunology*
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Viral Proteins / metabolism
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Viral Structural Proteins / genetics
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Viral Structural Proteins / immunology*
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Viral Structural Proteins / metabolism
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Viral Vaccines / genetics
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Viral Vaccines / immunology
Substances
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Antibodies, Viral
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Immunodominant Epitopes
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Phosphoproteins
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Recombinant Fusion Proteins
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Viral Proteins
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Viral Structural Proteins
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Viral Vaccines
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p54 protein, African swine fever virus
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P30 protein, African Swine Fever Virus