Abstract
We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide "barcodes." We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1. Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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CCAAT-Binding Factor / genetics
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CCAAT-Binding Factor / metabolism
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DNA Ligase ATP
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DNA Ligases / genetics
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DNA Ligases / metabolism
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DNA Repair*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Genes, Fungal*
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Genetic Complementation Test
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Mutation*
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Nucleic Acid Hybridization
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Oligonucleotide Array Sequence Analysis*
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Plasmids
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Recombination, Genetic*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / physiology
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae Proteins / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transformation, Genetic
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Two-Hybrid System Techniques
Substances
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CCAAT-Binding Factor
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DNA-Binding Proteins
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Fungal Proteins
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HAP3 protein, S cerevisiae
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LIF1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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DNA Ligases
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DNA Ligase ATP