Abstract
A chromosomally located beta-lactamase gene, cloned and expressed in Escherichia coli from a reference strain of the enterobacterial species Kluyvera cryocrescens, encoded a clavulanic acid-inhibited Ambler class A enzyme, KLUC-1, with a pI value of 7.4. KLUC-1 shared 86% amino acid identity with a subgroup of plasmid-mediated CTX-M-type extended-spectrum beta-lactamases (CTX-M-1, -3, -10, -11, and -12), the most closely related enzymes, and 77% amino acid identity with KLUA-1 from Kluyvera ascorbata. The substrate profile of KLUC-1 corresponded to that of CTX-M-type enzymes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anti-Bacterial Agents / pharmacology
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Cephalosporins / metabolism
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Chromosomes, Bacterial / genetics*
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Cloning, Molecular
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DNA, Bacterial / genetics
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Enterobacteriaceae / drug effects
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Enterobacteriaceae / enzymology
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Enterobacteriaceae / genetics*
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Genes, Bacterial / genetics
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Kinetics
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Open Reading Frames / genetics
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Plasmids / genetics
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Substrate Specificity
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Cephalosporins
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DNA, Bacterial
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beta-Lactamases