Nonculture prediction of Neisseria meningitidis susceptibility to penicillin

A Antignac; J M Alonso; M K Taha

doi:10.1128/AAC.45.12.3625-3628.2001

Nonculture prediction of Neisseria meningitidis susceptibility to penicillin

Antimicrob Agents Chemother. 2001 Dec;45(12):3625-8. doi: 10.1128/AAC.45.12.3625-3628.2001.

Authors

A Antignac¹, J M Alonso, M K Taha

Affiliation

¹ Unité des Neisseria, Centre National de Référence des Méningocoques, Institut Pasteur, 75724 Paris Cedex 15, France.

Abstract

We developed a nonculture method to predict the susceptibility of Neisseria meningitidis to penicillin G. The penA gene was amplified and submitted to restriction fragment length polymorphism analysis. This approach was first validated with a collection of 75 meningococcal strains of known phenotypes. It was next successfully applied to 29 clinical samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Electrophoresis, Agar Gel
Genes, Bacterial / genetics
Microbial Sensitivity Tests
Neisseria meningitidis / drug effects*
Neisseria meningitidis / genetics
Penicillin G / pharmacology*
Penicillin Resistance
Penicillins / pharmacology*
Polymorphism, Restriction Fragment Length
Predictive Value of Tests
Reverse Transcriptase Polymerase Chain Reaction

Substances

DNA, Bacterial
Penicillins
Penicillin G