Abstract
Ordered differential display PCR was used to identify differentially expressed genes in rat dorsal root ganglia at 7 days following chronic constriction injury (CCI) of the sciatic nerve. Fourteen differentially displayed cDNA bands were isolated, cloned and verified by RT-PCR. The four mRNAs were increased, which included mRNAs encoding heat shock protein 27, fatty acid binding protein, apolipoprotein D and one novel gene. Six down-regulated clones were microtubule-associated protein 1B, protein tyrosine phosphatase alpha, Kv1.2 channel, myelin protein SR13, medium-sized neurofilament protein, and one novel gene. Our results show that many differentially regulated genes after CCI may play a role in nerve degeneration and/or regeneration and provide a molecular framework for understanding the peripheral mechanism underlying neuropathic pain.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apolipoproteins / genetics
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Apolipoproteins D
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Base Sequence / physiology
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Carrier Proteins / genetics
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Down-Regulation / genetics
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Fatty Acid-Binding Protein 7
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Fatty Acid-Binding Proteins
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Ganglia, Spinal / injuries
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Ganglia, Spinal / metabolism*
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Ganglia, Spinal / physiopathology
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Gene Expression Profiling
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Gene Expression Regulation / physiology*
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HSP27 Heat-Shock Proteins
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Heat-Shock Proteins*
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Kv1.2 Potassium Channel
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Male
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Microtubule-Associated Proteins / genetics
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Molecular Sequence Data
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Myelin Proteins / genetics
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Neoplasm Proteins / genetics
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism
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Neuralgia / genetics*
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Neuralgia / metabolism
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Neuralgia / physiopathology
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Neurofilament Proteins / genetics
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Peripheral Nervous System Diseases / genetics*
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Peripheral Nervous System Diseases / metabolism
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Peripheral Nervous System Diseases / physiopathology
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Polymerase Chain Reaction
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Potassium Channels / genetics
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Potassium Channels, Voltage-Gated*
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Protein Tyrosine Phosphatases / genetics
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RNA, Messenger / metabolism*
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Rats
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Rats, Sprague-Dawley
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Up-Regulation / genetics
Substances
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Apolipoproteins
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Apolipoproteins D
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Carrier Proteins
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Fabp7 protein, rat
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Fatty Acid-Binding Protein 7
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Fatty Acid-Binding Proteins
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HSP27 Heat-Shock Proteins
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Heat-Shock Proteins
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Hspb1 protein, rat
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Kcna2 protein, rat
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Kv1.2 Potassium Channel
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Microtubule-Associated Proteins
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Myelin Proteins
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Neoplasm Proteins
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Nerve Tissue Proteins
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Neurofilament Proteins
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Pmp22 protein, mouse
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Pmp22 protein, rat
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Potassium Channels
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Potassium Channels, Voltage-Gated
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RNA, Messenger
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microtubule-associated protein 1B
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neurofilament protein M
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Protein Tyrosine Phosphatases