Abstract
An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.
MeSH terms
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Amino Acid Sequence
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Antibodies / immunology
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Cell Fractionation
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Centrifugation / methods
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Endoribonucleases / immunology
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Endoribonucleases / metabolism
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Macromolecular Substances
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Molecular Sequence Data
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Precipitin Tests
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RNA Helicases / metabolism
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RNA Processing, Post-Transcriptional
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RNA, Messenger / metabolism*
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Rhodobacter capsulatus / chemistry
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Rhodobacter capsulatus / genetics
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Rhodobacter capsulatus / metabolism*
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Sequence Analysis, Protein
Substances
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Antibodies
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Macromolecular Substances
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RNA, Messenger
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Endoribonucleases
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ribonuclease E
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RNA Helicases