Peroxidase(POX) isozyme analysis of Gastrodia elata f. glaucca, G. elata f. elata and its hybrid were carried out. The results indicated that shoot apex meristem was optimal material for POX analysis and the determination of the hybrid. The zymogram hands were stable and clear. POX isozyme zymogram of the hybrid F1(H x W) represented the visible supplement relation between Gastrodia elata f. elata and Gastrodia elata f. glaucca.