[Expressions of fibronectin and laminin in the middle ear mucosa with experimental middle ear effusions: immunohistochemical study]

Zhonghua Er Bi Yan Hou Ke Za Zhi. 1998 Jun;33(3):172-4.
[Article in Chinese]

Abstract

Objective: To understand the role of two components of extracellular matrix: fibronectin (FN) and laminin (LN) in the proliferated mucosa of otitis media with effusions.

Methods: A model of otitis media with effusions was established by coagulating the orifices of the eustachian tube under an endoscope in 30 guinea pigs. The expressions of FN and LN in the proliferated mucosa from these ears were detected using Streptavidin peroxidase (SP) method.

Results: In 60 ears with the orifices of the eustachinan tube coagulated, 28 ears showed otitis media with effusions. The increased expressions of FN on the fibroblasts and LN on the basement membranes and neovasculations were noted in the ears with otitis media with effusions.

Conclusion: FN and LN may involve in the proliferated response of middle ear mucosa with otitis media with effusions. It may play a certain role in the occurrence of middle ear mucosa proliferations and adhesions.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Basement Membrane / metabolism
  • Ear, Middle / metabolism*
  • Fibroblasts / metabolism
  • Fibronectins / metabolism*
  • Guinea Pigs
  • Immunohistochemistry
  • Laminin / metabolism*
  • Neovascularization, Pathologic
  • Otitis Media with Effusion / etiology
  • Otitis Media with Effusion / metabolism*
  • Random Allocation

Substances

  • Fibronectins
  • Laminin