New method for evaluation of virucidal activity of antiseptics and disinfectants

Appl Environ Microbiol. 2001 Dec;67(12):5844-8. doi: 10.1128/AEM.67.12.5844-5848.2001.

Abstract

Counting culturable viruses adsorbed to cellulose nitrate filters (the VIRADEN method) is proposed as a simple procedure for the evaluation of the virucidal activity of antiseptics and disinfectants. The virucidal activities of two different doses of iodine, chlorine, glutaraldehyde, and chlorhexidine digluconate on poliovirus 1 were tested with a standardized procedure and with the VIRADEN method. The two procedures assayed provided similar results.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents, Local / pharmacology*
  • Chlorhexidine / analogs & derivatives*
  • Chlorhexidine / pharmacology
  • Chlorine / pharmacology
  • Disinfectants / pharmacology*
  • Humans
  • Iodine / pharmacology
  • Microbial Sensitivity Tests / methods
  • Poliovirus / drug effects*
  • Poliovirus / growth & development
  • Viral Plaque Assay

Substances

  • Anti-Infective Agents, Local
  • Disinfectants
  • Chlorine
  • Iodine
  • chlorhexidine gluconate
  • Chlorhexidine