Apoptosis induction associated with cell cycle dysregulation by rice bran agglutinin

N Miyoshi; Y Koyama; Y Katsuno; S Hayakawa; T Mita; T Ohta; K Kaji; M Isemura

doi:10.1093/oxfordjournals.jbchem.a003051

Apoptosis induction associated with cell cycle dysregulation by rice bran agglutinin

J Biochem. 2001 Dec;130(6):799-805. doi: 10.1093/oxfordjournals.jbchem.a003051.

Authors

N Miyoshi¹, Y Koyama, Y Katsuno, S Hayakawa, T Mita, T Ohta, K Kaji, M Isemura

Affiliation

¹ Laboratory of Cellular Biochemistry, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Yada, Shizuoka 422-8526, Japan.

PMID: 11726280
DOI: 10.1093/oxfordjournals.jbchem.a003051

Abstract

Effects of rice bran agglutinin (RBA) on human monoblastic leukemia U937 cells were examined in comparison with those of wheat germ agglutinin (WGA) and Viscum album agglutinin (VAA). These lectins inhibit cell growth, and several lines of evidence indicate that the growth inhibition is caused by the induction of apoptosis. We observed that RBA induces chromatin condensation, externalization of membrane phosphatidylserine, and DNA ladder formation, features of apoptosis. DNA ladder formation was inhibited by a general inhibitor against caspases, which are known to play essential roles in apoptosis. Flow cytometric analysis revealed that RBA and WGA cause G2/M phase cell cycle arrest with increased expression of Waf1/p21, while cell cycle arrest was not observed for VAA. These data indicate that RBA induces apoptosis associated with cell cycle arrest in U937 cells, and suggest that the induction mechanism for RBA is similar to that for WGA, but different from that for VAA.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis*
Cell Cycle / drug effects
Cell Division / drug effects
Chromatin / drug effects*
Cyclin-Dependent Kinase Inhibitor p21
Cyclins / agonists*
Cyclins / biosynthesis
Cysteine Proteinase Inhibitors / pharmacology
DNA Fragmentation / drug effects*
G2 Phase / drug effects
Humans
Lectins / pharmacology*
Lymphoma, Large B-Cell, Diffuse
Mitosis / drug effects
Phosphatidylserines / metabolism
Plant Lectins*
Plant Preparations*
Plant Proteins / pharmacology*
Ribosome Inactivating Proteins, Type 2
Toxins, Biological / pharmacology
U937 Cells
Wheat Germ Agglutinins / pharmacology

Substances

CDKN1A protein, human
Chromatin
Cyclin-Dependent Kinase Inhibitor p21
Cyclins
Cysteine Proteinase Inhibitors
Lectins
Phosphatidylserines
Plant Lectins
Plant Preparations
Plant Proteins
Ribosome Inactivating Proteins, Type 2
Toxins, Biological
Wheat Germ Agglutinins
ribosome inactivating protein, Viscum
rice lectin