Abstract
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD; dioxin), a member of a class of environmental pollutants represented by polychlorinated dibenzo-p-dioxins and dibenzofurans, is one of the most toxic artificial compounds ever developed. In this study, we identified a novel TCDD target gene, DIF-3 (dioxin inducible factor-3), by cDNA representational difference analysis. DIF-3 protein is a nuclear factor and possesses a zinc-finger motif at its N-terminus. High DIF-3 mRNA expression in the testes was demonstrated by Northern blot analysis and abundant DIF-3 protein was detected during spermatogenesis. Thus, these results suggest that DIF-3 may be a target gene mediating the reproductive toxicity induced by TCDD.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Blotting, Northern
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Blotting, Western
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Cell Line
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DNA, Complementary
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Expressed Sequence Tags
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Fluorescent Antibody Technique, Indirect
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Gene Expression Profiling
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Gene Expression Regulation, Developmental / drug effects*
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Male
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Mice
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Molecular Sequence Data
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Molecular Weight
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Nuclear Proteins / analysis
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics*
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Nuclear Proteins / physiology
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Oligonucleotide Array Sequence Analysis
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Polychlorinated Dibenzodioxins / pharmacology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Spermatogenesis*
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Stem Cells
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Testis / metabolism
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Zinc Fingers
Substances
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DNA, Complementary
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Nuclear Proteins
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Polychlorinated Dibenzodioxins
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RNA, Messenger