Abstract
A mini-Tn5 transposon derivative, mini-Tn5cyaA', has been constructed. It contains a promoter-less and ribosome binding site-deficient reporter gene, encoding the catalytic domain of Bordetella pertussis adenylate cyclase toxin (CyaA'). We used this system to mutagenize B. bronchiseptica and we developed a screen for identification of mutants containing cyaA' translational fusions. This system was used to identify B. bronchiseptica genes that encode surface-exposed and secreted proteins.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenylate Cyclase Toxin
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Base Sequence
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Bordetella bronchiseptica / genetics*
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Bordetella bronchiseptica / metabolism
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DNA Transposable Elements*
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Genes, Reporter
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism*
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Molecular Sequence Data
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Mutagenesis, Insertional*
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Protein Biosynthesis
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Protein Precursors / genetics
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Protein Precursors / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
Substances
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Adenylate Cyclase Toxin
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Bacterial Proteins
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DNA Transposable Elements
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Membrane Proteins
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Protein Precursors
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Recombinant Fusion Proteins