Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterants

Planta Med. 2001 Nov;67(8):781-3. doi: 10.1055/s-2001-18340.

Abstract

A 420-bp RAPD fragment from Panax quinquefolius was converted to a sequence characterized amplified region (SCAR) marker. The main difference between the SCAR of P. quinquefolius and its homolog in P. ginseng is the presence of a 25 bp insertion in the latter. Primers derived from this sequence were successfully used to authenticate six Panax species and two common adulterants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers
  • DNA, Plant
  • Drug Contamination
  • Drugs, Chinese Herbal
  • Molecular Sequence Data
  • Panax / classification
  • Panax / genetics*
  • Plant Roots
  • Random Amplified Polymorphic DNA Technique / methods*
  • Sequence Alignment

Substances

  • DNA Primers
  • DNA, Plant
  • Drugs, Chinese Herbal