IL-1beta-induced expression of matrix metalloproteinases and gliostatin/platelet-derived endothelial cell growth factor (GLS/PD-ECGF) in a chondrosarcoma cell line (OUMS-27)

Y Ieda; Y Waguri-Nagaya; T Iwahasi; T Otsuka; N Matsui; M Namba; K Asai; T Kato

doi:10.1007/s002960100129

IL-1beta-induced expression of matrix metalloproteinases and gliostatin/platelet-derived endothelial cell growth factor (GLS/PD-ECGF) in a chondrosarcoma cell line (OUMS-27)

Rheumatol Int. 2001 Oct;21(2):45-52. doi: 10.1007/s002960100129.

Authors

Y Ieda¹, Y Waguri-Nagaya, T Iwahasi, T Otsuka, N Matsui, M Namba, K Asai, T Kato

Affiliation

¹ Department of Orthopedic Surgery, Nagoya City University Medical School, Japan. [email protected]

PMID: 11732857
DOI: 10.1007/s002960100129

Abstract

The purpose of this study was to examine how chondrocytes are involved in the molecular mechanism of inflammation in rheumatoid arthritis (RA). A chondrosarcoma cell line (OUMS-27) was cultured and treated with interleukin-1beta (IL-1beta). Changes in the expression levels of matrix metalloproteinase-1 (MMP-1), metalloproteinase-13 (MMP-13), and gliostatin/platelet-derived endothelial cell growth factor (GLS/PD-ECGF) were assessed by reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assays. IL-1beta induced the expressions of MMP-1, MMP-13, and GLS mRNAs and proteins in a dose-dependent manner. Selective inhibition of the p38 mitogen-activated protein kinase (p38 MAPK) pathway with SB 203580 and SB 202190 blocked the expression of MMP-1, MMP-13, and GLS more strongly than selective in hibition of the extracellular signal-regulated kinase 1 and 2 (ERK1/2) pathway by PD 98059. These findings suggest that chondrocytes may intensify cartilage destruction and inflammation in RA by the induction of MMP-1, MMP-13, and GLS by IL-1beta and that the p38 MAPK pathway plays an important role in these inductions.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Chondrosarcoma / pathology
Chondrosarcoma / physiopathology
Collagenases / drug effects
Collagenases / genetics*
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Gene Expression / physiology
Humans
Interleukin-1 / metabolism*
Interleukin-1 / pharmacology
Matrix Metalloproteinase 1 / drug effects
Matrix Metalloproteinase 1 / genetics*
Matrix Metalloproteinase 13
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases / antagonists & inhibitors
Mitogen-Activated Protein Kinases / metabolism*
Molecular Sequence Data
RNA, Messenger / analysis
Reference Values
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Thymidine Phosphorylase / drug effects
Thymidine Phosphorylase / genetics*
Thymidine Phosphorylase / metabolism
Tumor Cells, Cultured
p38 Mitogen-Activated Protein Kinases

Substances

Interleukin-1
RNA, Messenger
Thymidine Phosphorylase
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases
p38 Mitogen-Activated Protein Kinases
Collagenases
MMP13 protein, human
Matrix Metalloproteinase 13
Matrix Metalloproteinase 1