Matrix-bound fibroblasts regulate angiogenesis by modulation of VE-cadherin

Eur J Clin Invest. 2001 Nov;31(11):931-8. doi: 10.1046/j.1365-2362.2001.00914.x.

Abstract

Background: Vascular endothelial cadherin (VE-cadherin/cadherin-5) has previously been described as playing a specific role in angiogenesis due to its localisation at areas of intercellular contact, where it functions in maintenance of tubular architecture. Matrix-bound fibroblasts have been show to produce a number of factors that are important in inducing angiogenesis and to promote tubule-formation by human endothelial cells, an indicator of angiogenic potential.

Results: Tubule formation stimulated by fibroblast-derived growth factors can be prevented by the addition of monoclonal antibody to VE-cadherin. In addition, fibroblasts-derived growth factors are able to modulate the expression and hence the regulation of this endothelial cell specific cadherin.

Conclusions: The change in VE-cadherin expression of human endothelial cells by fibroblast-derived growth factors may contribute to the regulation of angiogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD
  • Blotting, Western
  • Cadherins / metabolism*
  • Cell Adhesion
  • Cells, Cultured
  • Coculture Techniques
  • Culture Media, Conditioned
  • Culture Media, Serum-Free
  • Cytoskeletal Proteins / metabolism
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism
  • Fibroblasts / metabolism*
  • Humans
  • Immunohistochemistry
  • Neovascularization, Physiologic*
  • Octoxynol
  • Reverse Transcriptase Polymerase Chain Reaction
  • Trans-Activators*
  • beta Catenin

Substances

  • Antigens, CD
  • CTNNB1 protein, human
  • Cadherins
  • Culture Media, Conditioned
  • Culture Media, Serum-Free
  • Cytoskeletal Proteins
  • Trans-Activators
  • beta Catenin
  • cadherin 5
  • Octoxynol