Abstract
We have isolated a full-length cDNA clone that encodes for a Schizosaccharomyces pombe homolog of the dual-specificity protein kinase of the LAMMER family, lkh1 (lammer kinase homolog). The proposed Lkh1 protein contains 575 amino acids. The lkh1(+) null mutant is viable, but exhibits flocculation upon reaching stationary phase in liquid media and filamentous adhesion growth on solid media. Analysis of the flocculation activity of the lkh1(+) null mutant indicates that asexual aggregation of S. pombe cells into floccules is divalent cation-dependent and galactose-specific. We also demonstrate that the Saccharomyces cerevisiae LAMMER kinase homolog, Kns1, can substitute for the Lkh1 function in S. pombe.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cations, Divalent / pharmacology
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Cell Adhesion
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DNA, Complementary / genetics
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DNA, Fungal / genetics
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Flocculation
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Galactose / pharmacology
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Gene Deletion
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Genes, Fungal
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Molecular Sequence Data
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Protein Serine-Threonine Kinases
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces / drug effects
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Schizosaccharomyces / enzymology*
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Schizosaccharomyces / genetics
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Schizosaccharomyces / growth & development*
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Schizosaccharomyces pombe Proteins*
Substances
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Cations, Divalent
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DNA, Complementary
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DNA, Fungal
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Fungal Proteins
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KNS1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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Protein Kinases
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lkh1 protein, S pombe
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Protein Serine-Threonine Kinases
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Galactose