Abstract
Lipopolysaccharide from a wbjA mutant, deficient in a putative glycosyltransferase from Pseudomonas aeruginosa serogroup O11, was compared to that from an O-antigen polymerase mutant. Results suggest that WbjA adds the terminal glucose to complete the serogroup O11 O-antigen unit and identifies the biological repeating unit as [-2)-beta-D-glucose-(1-3)-alpha-L-N-acetylfucosamine-(1-3)-beta-D-N-acetylfucosamine-(1].
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins*
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Carbohydrate Sequence
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Glucose / metabolism*
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Glucosyltransferases / metabolism*
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Molecular Sequence Data
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O Antigens / biosynthesis*
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Pseudomonas aeruginosa / immunology
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Pseudomonas aeruginosa / metabolism*
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Sequence Analysis
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Bacterial Proteins
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O Antigens
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Pseudomonas aeruginosa O11 antigen
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Glucosyltransferases
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WbjA protein, Pseudomonas aeruginosa
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Glucose