Abstract
dMi-2, the ATPase subunit of the Drosophila nucleosome remodelling and histone deacetylation (dNuRD) complex, was identified in a two-hybrid screen as an interacting partner of the transcriptional repressor, Tramtrack69 (Ttk69). A short region of Ttk69 is sufficient to mediate this interaction. Ttk69, but not the Ttk88 isoform, co-purifies with the dNuRD complex isolated from embryo extracts. dMi-2 and Ttk69 co-immunoprecipitate from embryonic extracts, indicating that they can associate in vivo. Both dMi-2 and Ttk69 co-localize at a number of discrete sites on polytene chromosomes, showing that they bind common target loci. We also demonstrate that dMi-2 and Ttk interact genetically, indicating a functional interaction in vivo. We propose that Ttk69 represses some target genes by remodelling chromatin structure through the recruitment of the dNuRD complex.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases*
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Animals
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Autoantigens / genetics
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Autoantigens / metabolism*
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Blotting, Western
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Chromatin / chemistry
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Chromatin / genetics
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Chromatin / metabolism*
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Drosophila Proteins*
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Drosophila* / genetics
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Drosophila* / metabolism
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Gene Expression Regulation
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Histone Deacetylases / chemistry*
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Histone Deacetylases / metabolism*
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Mi-2 Nucleosome Remodeling and Deacetylase Complex
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Protein Binding
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Protein Subunits
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Two-Hybrid System Techniques
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Yeasts
Substances
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Autoantigens
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Carrier Proteins
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Chromatin
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Drosophila Proteins
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Mi-2 protein, Drosophila
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Protein Subunits
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Repressor Proteins
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ttk protein, Drosophila
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Histone Deacetylases
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Mi-2 Nucleosome Remodeling and Deacetylase Complex
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Adenosine Triphosphatases