Methylated mono- and di(ethylene glycol)-functionalized beta-sheet forming polypeptides

Biomacromolecules. 2001 Spring;2(1):17-21. doi: 10.1021/bm005597p.

Abstract

We have synthesized methylated mono- and di(ethylene glycol)-functionalized polymers of L-serine and L-cysteine that adopt beta-sheet conformations in the solid state: poly(O-(2-(2-methoxyethoxy)ethyl)-L-serine), poly(1); poly(O-(2-(methoxy)ethyl)-L-serine), poly(2); and poly(S-(2-(2-methoxyethoxy)ethoxy)carbonyl-L-cysteine), poly(3). Of these three polymers, only poly(1) was found to be highly soluble in water independent of pH. Circular dichroism analysis of poly(1) in water or trifluoroethanol at 25 degrees C revealed that it is in a random conformation, which was unperturbed by changes in pH, buffer, or temperature. However, addition of methanol or acetonitrile to aqueous solutions of poly(1) resulted in a transition to the beta-sheet conformation, as found in the solid state. The polymers were synthesized by transition metal catalyzed polymerization of amino acid-N-carboxyanhydrides, prepared from the functionalized amino acids and represent a new class of readily processable beta-sheet forming polypeptides.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Buffers
  • Circular Dichroism
  • Cysteine / analogs & derivatives*
  • Cysteine / chemistry*
  • Hydrogen-Ion Concentration
  • Molecular Structure
  • Peptides / chemical synthesis
  • Peptides / chemistry*
  • Protein Structure, Secondary
  • Serine / analogs & derivatives*
  • Serine / chemistry*
  • Solubility
  • Solutions
  • Temperature

Substances

  • Buffers
  • Peptides
  • Solutions
  • Serine
  • Cysteine